LS-C152798

antibody from LSBio

Targeting: TEK CD202b, TIE-2, TIE2, VMCM, VMCM1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C152798

Provider

LSBio

Product name

TEK / TIE2 Antibody (phospho-Tyr992) LS-C152798

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WB: Untreated and pervanadate-treated HUVEC (lanes 1 and 2, respectively) were resolved by SDS-PAGE, transferred to PVDF (Immobilon-P), and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated HUVEC lysate was either unblocked, TIE2 Tyr992 phosphorylated-peptide blocked, or TIE2 non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-TIE2 (Tyr992) (1:1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated TIE2.