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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
- Functional assay [1]
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- Product number
- 102-PA111 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- TIE-2
- Antibody type
- Polyclonal
- Antigen
- Recombinant human sTIE-2
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITI
GRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREK
ASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPA
TLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFI
HSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGN
LFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGV
CHEDTGECICPPGFMGRTCEKACELHTFGRTCKER
CSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEAC
HPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQG
LQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKA
SGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVA
IFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVK
VLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIK
SKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTE
YELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPR
GLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRS
VQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARV
NTKAQGEWSEDLTAWTLSDILPPQPENIKISNITH
SSAVISWTILDGYSISSITIRYKVQGKNEDQHVDV
KIKNATIIQYQLKGLEPETAYQVDIFAENNIGSSN
PAFSHELVTLPESQAPADLGGGKTRHHHHHH- Antibody clone number
- Rabbit IG
- Vial size
- 200 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human and mouse sTIE-1 and sTIE-2 using a polyclonal antibody directed against human recombinant sTIE-2. There is a strong cross reactivity with mouse sTIE-2 but not with human and mouse sTIE-1 visible.
- Sample type
- HDLEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Functional ELISA with anti-human TIE-2 [Cat# 102-PA111]. Recombinant human soluble TIE-2 [Cat# S01-043] was coated with increasing amounts on a 96 well microtiter plate.
