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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF928 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Tie-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects zebrafish Tie-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Tie-2, recombinant mouse Tie-2, and rhTie-1 is observed.
- Reactivity
- Zebrafish
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Tie-2 in Zebrafish Embryo. Tie-2 was detected in immersion fixed whole mount zebrafish embryo (24 hours old) using 10 µg/mL Goat Anti-Zebrafish Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF928) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
