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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB928 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Tie-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects zebrafish Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human or mouse Tie-1 and Tie-2 is observed.
- Reactivity
- Zebrafish
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A crucial role of caldesmon in vascular development in vivo.
Zheng PP, Severijnen LA, van der Weiden M, Willemsen R, Kros JM
Cardiovascular research 2009 Feb 1;81(2):362-9
Cardiovascular research 2009 Feb 1;81(2):362-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Angiopoietin-2 binding to Tie-2 blocked by Zebrafish Tie-2 Antibody. In a functional ELISA, Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 μg/mL, this antibody will block >90% of the binding.
