Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations [0]
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- Product number
- 14-2029-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells. Applications Reported: This 33.1 antibody has been reported for use in western blotting. Applications Tested: This 33.1 antibody has been tested by immunoblotting of reduced cell lysate prepared from the bEND3 cell line at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 33.1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.
Angiopoietin-1-induced ubiquitylation of Tie2 by c-Cbl is required for internalization and degradation.
Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2.
Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver.
Coffelt SB, Chen YY, Muthana M, Welford AF, Tal AO, Scholz A, Plate KH, Reiss Y, Murdoch C, De Palma M, Lewis CE
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90
Angiopoietin-1-induced ubiquitylation of Tie2 by c-Cbl is required for internalization and degradation.
Wehrle C, Van Slyke P, Dumont DJ
The Biochemical journal 2009 Oct 12;423(3):375-80
The Biochemical journal 2009 Oct 12;423(3):375-80
Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2.
Nguyen VP, Chen SH, Trinh J, Kim H, Coomber BL, Dumont DJ
BMC cell biology 2007 Mar 6;8:10
BMC cell biology 2007 Mar 6;8:10
Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver.
Hsu HC, Ema H, Osawa M, Nakamura Y, Suda T, Nakauchi H
Blood 2000 Dec 1;96(12):3757-62
Blood 2000 Dec 1;96(12):3757-62
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