MA5-29596

antibody from Invitrogen Antibodies

Targeting: TEK CD202b, TIE-2, TIE2, VMCM, VMCM1

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29596

Provider

Invitrogen Antibodies

Product name

TEK Recombinant Rabbit Monoclonal Antibody (116)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

116

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with TEK Recombinant Rabbit Monoclonal Antibody (116) (Product # MA5-29596, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with TEK Recombinant Rabbit Monoclonal Antibody (116) (Product # MA5-29596, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human TEK expression on HUVEC cells. Cells were stained with TEK Recombinant Rabbit Monoclonal Antibody (116) (Product # MA5-29596), then a FITC-conjugated Secondary antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.