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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-69140 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAMTS4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.
Zhang M, Zhang R, Zheng T, Chen Z, Ji G, Peng F, Wang W
Frontiers in pharmacology 2021;12:680585
Frontiers in pharmacology 2021;12:680585
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIGURE 4 XH attenuated ECM degradation in IL-1beta-treated chondrocytes. (A) The immunofluorescence study of MMP-13 in IL-1beta-treated chondrocytes. (B) The summary data of fluorescence intensity of MMP-13. (C) The proteins expression of MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, collagen-II, and aggrecan were detected by western blotting. (D-I) were the quantified values of tested proteins. All experiments were performed in triplicate and data are presented as the mean +- standard deviation. * p < 0.05 and ** p < 0.01. NC, negative control; Low, XH (5 muM); High, XH (20 muM).
