Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- NB500-378 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-378, RRID:AB_10001195
- Product name
- Mouse Monoclonal Fc gamma RIII (CD16) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-154 reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes. HLDA V
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab.
The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa.
Gasdaska JR, Sherwood S, Regan JT, Dickey LF
Molecular immunology 2012 Mar;50(3):134-41
Molecular immunology 2012 Mar;50(3):134-41
The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
Tamm A, Schmidt RE
Journal of immunology (Baltimore, Md. : 1950) 1996 Aug 15;157(4):1576-81
Journal of immunology (Baltimore, Md. : 1950) 1996 Aug 15;157(4):1576-81
A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa.
de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE
Journal of immunology (Baltimore, Md. : 1950) 1996 Apr 15;156(8):2948-55
Journal of immunology (Baltimore, Md. : 1950) 1996 Apr 15;156(8):2948-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Flow (Cell Surface): Fc gamma RIIIA/CD16a Antibody (MEM-154) [Azide Free] [NBP2-37713] - Surface staining of human peripheral blood cells with anti-human CD16 (MEM-154) PE. The antibody does not react with CD16a present on NK cells in many subjects. Image using the Azide Free form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Staining of human peripheral blood cells with anti-human CD16 (MEM-154) purified, GAM/APC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Flow cytometry surface staining pattern of human peripheral blood stained using Fc gamma RIII (CD16) Antibody (MEM-154) purified antibody (concentration in sample 2 ug/ml) GAM APC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).