Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-52644-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD16/CD32 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: CD16/CD32 Antibody (2.4G2) [NBP2-52644] - Staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/32-positive population is apparent.