Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB2546B-025 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Fc gamma RIII (CD16) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 25 Tests
- Storage
- Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2A Biotinylated Isotype Control (Catalog # IC003B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.