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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Product number
- NB500-523 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-523, RRID:AB_10000840
- Product name
- Mouse Monoclonal Fc gamma RIIIA/CD16a Antibody
- Antibody type
- Monoclonal
- Description
- Multi-step. The antibody MEM-168 reacts with CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA
- Reactivity
- Human, Porcine, Simian
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Surface staining of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03) Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168) Cells in the granulocyte gate were used for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 488 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody AF488. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 647 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Ima
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Human PBMC stained with Fc gamma RIIIA/CD16a antibody. Image from verified customer review.
