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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [3]
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- Product number
- GTX11909 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11909, RRID:AB_367301
- Product name
- ATF6 antibody [70B1413]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references 6-Shogaol induces cell cycle arrest and apoptosis in human hepatoma cells through pleiotropic mechanisms.
Wu JJ, Omar HA, Lee YR, Teng YN, Chen PS, Chen YC, Huang HS, Lee KH, Hung JH
European journal of pharmacology 2015 Sep 5;762:449-58
European journal of pharmacology 2015 Sep 5;762:449-58
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of ATF6 in NIH3T3 cell lysate using IMG-273 at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/cleavage product.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6. Lane 1, 293 cells transfected with full-length ATF6.* Lane 2, 293 cells transfected with partial length ATF6 (amino acids 1-373).* Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody, followed by an HRP-conjugated second step (20101) and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the ~90 kDa form of ATF6 described as full-length in the literature. *The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in mouse liver tissue enriched using 10088K using 3 ug/ml of IMG-273 (anti-ATF6) and 0.25 ug/ml (anti-GAPDH loading control). Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6* band may represent under glycosylated or cleaved/active ATF6.
