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LearnSM7007P
antibody from Acris Antibodies GmbH
Targeting: ATF6
ATF6A
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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- Product number
- SM7007P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM7007P, RRID:AB_973058
- Product name
- anti ATF6 alpha (full length + Cleaved Forms)
- Antibody type
- Monoclonal
- Antigen
- Partial protein containing amino acids 1-273 of Human ATF6.
- Reactivity
- Human, Mouse, Rat, Hamster, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 70B1413.1
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in NIH3T3 cell lysate using SM7007P ATF6 antibody at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/cleavage product.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in mouse liver tissue enriched using 10088K using 3 µg/ml of SM7007P ATF6 antibody and 0.25 µg/ml of anti-GAPDH Loading Control. Lane A contains 20 µgs of whole Mouse liver lysate, Lane B contains 20 µgs of total ER fraction, and Lane C contains 20 µgs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SM7007P ATF6 antibody.Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. Western blots were probed with 4 ug/ml of the monoclonal SM7007P ATF6 antibody followed by an HRP-conjugated second step and visualized with PicoTect Western Blot Chemiluminescence Substrate. Film was exposed for 1 min. The top arrow corresponds to the ~90 kDa form of ATF6 described as full-length in the literature. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).
