Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- PA5-72962 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in 1.0 mg/mL HeLa lysate. Samples were incubated in ATF6 polyclonal antibody (Product # PA5-72962). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in cell lysates of nuclear extracts. Samples were incubated in ATF6 polyclonal antibody (Product # PA5-72962). AICAR inhibits ER stress responses. AMPK-independent inhibition of human macrophage ER stress response by AICAR.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in 1.0 mg/mL HeLa lysate. Samples were incubated in ATF6 polyclonal antibody (Product # PA5-72962). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using ATF6 polyclonal antibody (Product # PA5-72962) conjugated with FITC (green). Counterstain was performed with DAPI (blue nuclei).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ATF6 in HeLa cells fixed and permeabilized for 10 minutes using -20 °C MeOH. Samples were incubated in ATF6 polyclonal antibody (Product # PA5-72962) using a dilution of 10 µg/mL overnight at 4 °C followed by anti-rabbit DyLight 488 (Green) with a dilution of 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.