Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-75478 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-75478, RRID:AB_11008989
- Product name
- Rabbit Polyclonal ATF6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.6 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome.
AMPK-independent inhibition of human macrophage ER stress response by AICAR.
Park SJ, Kim Y, Yang SM, Henderson MJ, Yang W, Lindahl M, Urano F, Chen YM
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 9;116(28):14154-14163
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 9;116(28):14154-14163
AMPK-independent inhibition of human macrophage ER stress response by AICAR.
Boß M, Newbatt Y, Gupta S, Collins I, Brüne B, Namgaladze D
Scientific reports 2016 Aug 26;6:32111
Scientific reports 2016 Aug 26;6:32111
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.