AF486

antibody from R&D Systems

Targeting: XCL1 ATAC, LPTN, LTN, SCM-1, SCM-1a, SCYC1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF486

Provider

R&D Systems

Product name

Mouse XCL1/Lymphotactin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GRO alpha , rhGRO beta , rhGRO gamma , and recombinant mouse MCP-5 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control.

Böttcher JP, Bonavita E, Chakravarty P, Blees H, Cabeza-Cabrerizo M, Sammicheli S, Rogers NC, Sahai E, Zelenay S, Reis e Sousa C
Cell 2018 Feb 22;172(5):1022-1037.e14

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Comprehensive assessment of chemokine expression profiles by flow cytometry.

Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23

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A parenteral DNA vaccine protects against pneumonic plague.

Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW
Vaccine 2010 Apr 19;28(18):3219-30

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody. Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND₅₀ is typically 5-15 µg/mL.