500-P40-1MG
antibody from Invitrogen Antibodies
Targeting: XCL1
ATAC, LPTN, LTN, SCM-1, SCM-1a, SCYC1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P40-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lymphotactin Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Lymphotactin (XCL1). Anti-Human Lymphotactin (XCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human Lymphotactin (XCL1) matrix. Sandwich ELISA: To detect Human Lymphotactin (XCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Lymphotactin (XCL1) (500-P40Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Lymphotactin (XCL1). Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLymphotactin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Enhanced expression of C chemokine lymphotactin in IgA nephropathy.
Ou ZL, Hotta O, Natori Y, Sugai H, Taguma Y, Natori Y
Nephron 2002 Jun;91(2):262-9
Nephron 2002 Jun;91(2):262-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human Lymphotactin (XCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Lymphotactin Polyclonal Antibody (Product # 500-P40-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Lymphotactin Polyclonal Antibody, Biotin (Product # 500-P40BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Lymphotactin (XCL1).