Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF695 - Provider product page
- Provider
- R&D Systems
- Product name
- Human XCL1/Lymphotactin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Ddetects human XCL1/Lymphotactin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P47992
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman BF, Fauci AS, Lusso P
PLoS pathogens 2013;9(12):e1003852
PLoS pathogens 2013;9(12):e1003852
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody. Recombinant Human XCL1/ Lymphotactin (Catalog # 695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dosdependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human XCL1/ Lymphotactin (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695). The ND50 is typically 10-30 µg/mL.