PA5-41992

antibody from Invitrogen Antibodies

Targeting: KHK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-41992

Provider

Invitrogen Antibodies

Product name

KHK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human fetal liver cell lysate using an anti-KHK polyclonal antibody (Product # PA5-41992).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-KHK Polyclonal Antibody (Product # PA5-41992) and a 32kDa and 37kDa band corresponding to Ketohexokinase were observed across tissues tested. Tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Rat Lung (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6) and Rat Kidney (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-KHK polyclonal antibody (Product # PA5-41992).