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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-30579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HGK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Rat (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.19 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HGK/MAP4K4 using 30 µg of Raji lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a HGK/MAP4K4 polyclonal antibody (Product # PA5-30579) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HGK Polyclonal Antibody (Product # PA5-30579). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HGK Polyclonal Antibody (Product # PA5-30579) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HGK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HGK Polyclonal Antibody (Product # PA5-30579) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HGK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HGK Polyclonal Antibody (Product # PA5-30579) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using MAP4K4 (Product # PA5-30579) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
