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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Immunohistochemistry [2]
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- Product number
- PA5-87769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LENGNNKAAS VQEEFMDAVC DGVGLGMKIL LDSGLEKAKE LQHELFRQCT KNEVTKEMKT NAMGLIRSLE NIFAESKIKS FRRQVQEENF EYQDFKRMVN RAPEFLKLKH CLEKAIEIII SALKGCHSDI NLLQTCVESI RNLASDFYSD FSVPSTSVGS YESRVTHIVH QELESLAKSL LFCFESEESP DLLKPWETYN QNTKEEHQQS KSSGIDGSKN KGVPKRVYEL HGSSPAVSSE ECTPSRIQWV; Positive Samples: HeLa, HL-60; Cellular Location: Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.46 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KIF14 was performed in L929 cells using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KIF14 was performed in U-2 OS cells using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KIF14 in L929 cells. Samples were incubated with KIF14 Polyclonal antibody (Product # PA5-87769) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KIF14 in U-2 OS cells. Samples were incubated with KIF14 Polyclonal antibody (Product # PA5-87769) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded rat ovary. Samples were incubated with KIF14 Polyclonal antibody (Product # PA5-87769) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded human breast cancer using. Samples were incubated with KIF14 Polyclonal antibody (Product # PA5-87769) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
