PAB3277

antibody from Abnova Corporation

Targeting: MAP3K20 MLK7, MLTK, MLTKalpha, MLTKbeta, MRK, ZAK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3277

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3277, RRID:AB_1577308

Product name

MLKLAK polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MLKLAK.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Oncogenic kinase signalling.

Blume-Jensen P, Hunter T
Nature 2001 May 17;411(6835):355-65

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Phosphoinositide 3-kinase signalling pathways.

Cantrell DA
Journal of cell science 2001 Apr;114(Pt 8):1439-45

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The RET proto-oncogene in human cancers.

Jhiang SM
Oncogene 2000 Nov 20;19(49):5590-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MLKLAK polyclonal antibody (Cat # PAB3277) in HepG2 cell line lysate (35 ug/lane). MLKLAK (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution). This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MLKLAK polyclonal antibody (Cat # PAB3277) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)