NBP1-90367
antibody from Novus Biologicals
Targeting: MAP3K20
MLK7, MLTK, MLTKalpha, MLTKbeta, MRK, ZAK
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-90367 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90367, RRID:AB_11006817
- Product name
- Rabbit Polyclonal ZAK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ZAK antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Liu J, McCleland M, Stawiski EW, Gnad F, Mayba O, Haverty PM, Durinck S, Chen YJ, Klijn C, Jhunjhunwala S, Lawrence M, Liu H, Wan Y, Chopra V, Yaylaoglu MB, Yuan W, Ha C, Gilbert HN, Reeder J, Pau G, Stinson J, Stern HM, Manning G, Wu TD, Neve RM, de Sauvage FJ, Modrusan Z, Seshagiri S, Firestein R, Zhang Z
Nature communications 2014 May 8;5:3830
Nature communications 2014 May 8;5:3830
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZAK Antibody [NBP1-90367] - Analysis in human cell line U-138 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZAK Antibody [NBP1-90367] - Analysis in human cell lines U2OS and MCF-7 using anti-AC013461.1 antibody. Corresponding AC013461.1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ZAK Antibody [NBP1-90367] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-90367] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.