GTX83459

antibody from GeneTex

Targeting: TYRO3 Brt, Dtk, Etk-2, Rek, RSE, Sky, Tif

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX83459

Provider

GeneTex

Proper citation

GeneTex Cat#GTX83459, RRID:AB_10729456

Product name

TYRO3 antibody [5B4]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

TYRO3 as a molecular target for growth inhibition and apoptosis induction in bladder cancer.

Dufour F, Silina L, Neyret-Kahn H, Moreno-Vega A, Krucker C, Karboul N, Dorland-Galliot M, Maillé P, Chapeaublanc E, Allory Y, Stransky N, Haegel H, Menguy T, Duong V, Radvanyi F, Bernard-Pierrot I
British journal of cancer 2019 Mar;120(5):555-564

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded lung tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-TYRO3 mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast tissue using anti-TYRO3 mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-TYRO3mouse monoclonal antibody. Heat-induced epitope retrieval by Tris-EDTA, pH8.0. (GTX83459, Dilution 1:50)