LS-C784245

antibody from LSBio

Targeting: TYRO3 Brt, Dtk, Etk-2, Rek, RSE, Sky, Tif

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C784245

Provider

LSBio

Product name

TYRO3 Antibody (clone OTI2D11, Carrier-free) LS-C784245

Antibody type

Monoclonal

Description

Affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2D11

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of TYRO3 by using monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-TYRO3 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of TYRO3 by using monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-TYRO3 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (Dilution 1:50).