PA5-22999

antibody from Invitrogen Antibodies

Targeting: TYRO3 Brt, Dtk, Etk-2, Rek, RSE, Sky, Tif

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22999

Provider

Invitrogen Antibodies

Product name

TYRO3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The target sequence has 94% sequence homology with rat.

Concentration

1.19 mg/mL

References

Submitted references

Cellular Targets and Receptor of Sexual Transmission of Zika Virus.

Bagasra O, Addanki KC, Goodwin GR, Hughes BW, Pandey P, McLean E
Applied immunohistochemistry & molecular morphology : AIMM 2017 Nov Dec;25(10):679-686

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TYRO3 using a polyclonal antibody (Product # PA5-22999).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TYRO3 in Lane 1: normal human brain; Lane 2: normal mouse brain. Samples were incubated in TYRO3 polyclonal antibody (Product # PA5-22999).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TYRO3 using a polyclonal antibody (Product # PA5-22999).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TYRO3 in U937 cells. Samples were incubated in TYRO3 polyclonal antibody (Product # PA5-22999) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TYRO3 in HeLa cells. Samples were incubated in TYRO3 polyclonal antibody (Product # PA5-22999) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 4. A, Dual-immunohistochemical staining of ZIKV and Tyro3 amino acid residue 100 to 250 antibodies: After incubation with primary antibodies against ZIKV and Tyro3, 2 different colors of secondary antibodies were utilized to detect and localize ZIKV-binding sites and Tyro3 expression on sperms. A, E, I, Sperms using light microscopy from the 3 semen specimens. B, F, J, The combined immunostains for ZIKV and Tyro3 expressions (bluish strain) photographed under a dual filter. C, G, K, The location of Tyro3 domain on the sperm's mid-piece. D, H, L, The location of ZIKV-binding site (yellow arrows) (magnification, x80). B, This insert represent a closer view of J, clearly showing the dual staining at the mid-piece of the spermatozoa. ZIKV indicates Zika virus.