Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- GTX23460 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23460, RRID:AB_385060
- Product name
- Adenosine A1 Receptor antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Altered expression of adenosine A1 and A2A receptors in the carotid body and nucleus tractus solitarius of adult male and female rats following neonatal caffeine treatment.
Bairam A, Joseph V, Lajeunesse Y, Kinkead R
Brain research 2009 Sep 1;1287:74-83
Brain research 2009 Sep 1;1287:74-83
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Supportive validation
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- Experimental details
- Immunohistochemistry analysis of Adenosine Receptor A1 showing positive staining in the cytoplasm of paraffin-treated Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Adenosine Receptor A1 polyclonal antibody (Product # GTX23460) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.