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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA3-041 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADORA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-041 detects Adenosine Receptor A1 from human and mouse samples. Reactivity is stronger in mouse, and is only recommended for use in overexpressed or recombinant human samples. PA3-041 has been successfully used in Western blot applications. By Western blot PA3-041 detects a ~34-37 kDa band representing Adenosine Receptor A1 from mouse brain. The PA3-041 immunogen is a mix of synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1 residues. The immunizing sequences are: C(309) Q P A P P I D E D L P E E R P D D(326) for human, and C(309) Q P K P P I E E D I P E E K A D D(326) for mouse.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Increased Signaling via Adenosine A1 Receptors, Sleep Deprivation, Imipramine, and Ketamine Inhibit Depressive-like Behavior via Induction of Homer1a.
Serchov T, Clement HW, Schwarz MK, Iasevoli F, Tosh DK, Idzko M, Jacobson KA, de Bartolomeis A, Normann C, Biber K, van Calker D
Neuron 2015 Aug 5;87(3):549-62
Neuron 2015 Aug 5;87(3):549-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection from mouse brain, using PA3-041.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Adenosine Receptor A1 was performed using 90% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (Product # PA3-041) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
