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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-27075 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADORA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Adenosine A1 Receptor using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Adenosine A1 Receptor polyclonal antibody (Product # PA5-27075) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADORA1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ADORA1 Polyclonal Antibody (Product # PA5-27075) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ADORA1 Polyclonal Antibody (Product # PA5-27075). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. ADORA1 Polyclonal Antibody (Product # PA5-27075) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
