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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA505727 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505727, RRID:AB_2623323
- Product name
- MDM4 (MDMX) mouse monoclonal antibody, clone OTI4D2 (formerly 4D2)
- Antibody type
- Monoclonal
- Description
- MDM4 (MDMX) mouse monoclonal antibody, clone OTI4D2 (formerly 4D2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MDM4
- Isotype
- IgG
- Antibody clone number
- OTI4D2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM4 (RC209620, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM4.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-MDM4 mouse monoclonal antibody (TA505727).
- Validation comment
- IF
