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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00000725-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000725-M01, RRID:AB_1136902
- Product name
- C4BPB monoclonal antibody (M01), clone 1G9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant C4BPB.
- Antigen sequence
MFFWCACCLMVAWRVSASDEHCPELPPVDNSIFVA
KEVEGQILGTYVCIKGYHLVGKKTLFCNASKEWDN
TTTECRLGHCPDPVLVNGEFSSSGPVNVSDKITFM
CNDHYILKGSNRSQCLEDHTWAPPFPICKSRDCDP
PGNPVHGYFEGNNFTLGSTISYYCEDRYYLVGVQE
QQCVDGEWSSALPVCKLIQEAPKPECEKALLAFQE
SKNLCEAMENFMQQLKESGMTMEELKYSLELKKAE
LKAKLL- Isotype
- IgG
- Antibody clone number
- 1G9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of the ovine complement 4 binding protein-beta (C4BPB) chain as a serum biomarker for enhanced diagnosis of sheep scab.
Wells B, Burgess ST, Nisbet AJ
Molecular and cellular probes 2013 Jun-Aug;27(3-4):158-63
Molecular and cellular probes 2013 Jun-Aug;27(3-4):158-63
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged C4BPB is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of C4BPB transfected lysate using anti-C4BPB monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with C4BPB MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
