Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-401-A19 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Product name
- Anti-ASPP2 (RABBIT) Antibody - 200-401-A19
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Cortactin-dependent control of Par1b-regulated epithelial cell polarity in Helicobacter infection.
Sharafutdinov I, Harrer A, Müsken M, Rottner K, Sticht H, Täger C, Naumann M, Tegtmeyer N, Backert S
Cell insight 2024 Jun;3(3):100161
Cell insight 2024 Jun;3(3):100161
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Protein A purified anti-ASPP2 to detect over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.
- Validation comment
- Western Blot