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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-38044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lefty1/Lefty2 Recombinant Rabbit Monoclonal Antibody (ARC2149)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HT-29, BxPC-3, Mouse brain, Rat brain
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC2149
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lefty1/Lefty2 using Lefty1/Lefty2 Recombinant Monoclonal Antibody (Product # MA5-38044) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lefty1/Lefty2 using Lefty1/Lefty2 Recombinant Monoclonal Antibody (Product # MA5-38044) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Lefty1/Lefty2 in human colon carcinoma tissue using Lefty1/Lefty2 Recombinant Monoclonal Antibody (Product # MA5-38044) at a dilution of 1:100.
