Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX122652 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX122652, RRID:AB_11167866
- Product name
- ROCK2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: ROCK2 protein stained by ROCK2 antibody (GTX122652) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 ?g of ROCK2 antibody (GTX122652).Western blot analysis was performed using ROCK2 antibody (GTX122652).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 £gg of ROCK2 antibody (GTX122652).Western blot analysis was performed using ROCK2 antibody (GTX122652).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ROCK2 antibody detects ROCK2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ROCK2 antibody (GTX122652) diluted at 1:500.