- GTX122652 - GeneTex ROCK2 antibody

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41710

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: ROCK2 protein stained by ROCK2 antibody (GTX122652) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 ?g of ROCK2 antibody (GTX122652).Western blot analysis was performed using ROCK2 antibody (GTX122652).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 £gg of ROCK2 antibody (GTX122652).Western blot analysis was performed using ROCK2 antibody (GTX122652).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 antibody detects ROCK2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ROCK2 antibody (GTX122652) diluted at 1:500.

GTX122652