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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319436 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Rock-2 phospho Y256 antibody (Phospho-specific)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Rock-2 phospho Y256 antibody (Phospho-specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ROCK2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-ROCK-2 pY256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
- Validation comment
- WB
