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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11303 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11303, RRID:AB_1713158
- Product name
- ROCK2 (phospho Y256) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ROCK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Rho GTPases: promising cellular targets for novel anticancer drugs.
Rho signalling at a glance.
Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Fritz G, Kaina B
Current cancer drug targets 2006 Feb;6(1):1-14
Current cancer drug targets 2006 Feb;6(1):1-14
Rho signalling at a glance.
Schwartz M
Journal of cell science 2004 Nov 1;117(Pt 23):5457-8
Journal of cell science 2004 Nov 1;117(Pt 23):5457-8
Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Takahashi N, Tuiki H, Saya H, Kaibuchi K
Genomics 1999 Jan 15;55(2):235-7
Genomics 1999 Jan 15;55(2):235-7
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- Experimental details
- Western blot using ROCK2 (phospho Y256) polyclonal antibody (Cat # PAB11303) shows detection of phosphorylated ROCK2 in transfected 293T cells.Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A.Lane 2 shows the lysate expressing WT ROCK2.The primary antibody was used at a 1 : 1000 dilution.Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
