600-401-998

antibody from Rockland Immunochemicals, Inc.

Targeting: ROCK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-998

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-998, RRID:AB_2182301

Product name

Anti-ROCK-2 (Rho-associated Protein Kinase-2) pY256 (RABBIT) Antibody - 600-401-998

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2 Contributes to Diabetes-Induced Vascular Dysfunction.

Akhtar S, Yousif MH, Dhaunsi GS, Sarkhouh F, Chandrasekhar B, Attur S, Benter IF
PloS one 2013;8(6):e67813

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OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3.

Herhaus L, Al-Salihi M, Macartney T, Weidlich S, Sapkota GP
Nature communications 2013;4:2519

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-ROCK-2 pY256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.

Validation comment

Western Blot