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- Antibody Data
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- Western blot [2]
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- Product number
- NB100-593 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-593, RRID:AB_10002027
- Product name
- Rabbit Polyclonal ROCK2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-593 is specific for human Rho Kinase alpha protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of V-ATPase recycling via a RhoA- and ROCKII-dependent pathway in epididymal clear cells.
Shum WW, Da Silva N, Belleannée C, McKee M, Brown D, Breton S
American journal of physiology. Cell physiology 2011 Jul;301(1):C31-43
American journal of physiology. Cell physiology 2011 Jul;301(1):C31-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.
