Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-21131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROCK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse brain tissue lysate. PA5-21131 can be used with blocking peptide PEP-1245.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Probing Anti-inflammatory Properties Independent of NF-κB Through Conformational Constraint of Peptide-Based Interleukin-1 Receptor Biased Ligands.
Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma.
Geranurimi A, Cheng CWH, Quiniou C, Zhu T, Hou X, Rivera JC, St-Cyr DJ, Beauregard K, Bernard-Gauthier V, Chemtob S, Lubell WD
Frontiers in chemistry 2019;7:23
Frontiers in chemistry 2019;7:23
Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma.
Thompson JM, Nguyen QH, Singh M, Pavesic MW, Nesterenko I, Nelson LJ, Liao AC, Razorenova OV
Oncogene 2017 Feb 23;36(8):1080-1089
Oncogene 2017 Feb 23;36(8):1080-1089
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain tissue lysate using a ROCK2 polyclonal antibody (Product # PA5-21131) at (A) 1 and (B) 2 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 Polyclonal Antibody (Product # PA5-21131) at (A) 1 and (B) 2 µg/mL
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 Polyclonal Antibody (Product # PA5-21131) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 Polyclonal Antibody (Product # PA5-21131) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Synthetic lethality of Y-27632 with VHL loss is mimicked by siRNA downregulation of ROCK1, not ROCK2 RCC4+-VHL matched cell lines were transfected with siRNAs targeting ROCK1, ROCK2, or non-targeting siRNA control (siControl). Twenty-four hours after transfection cells were plated for a clonogenic assay. Each transfection was done in triplicate, followed by clonogenic assays conducted in triplicate, and the experiments were repeated at least two times. ( a ) Transfection with siROCK1, but not siROCK2, resulted in significant reduction in RCC4 colony numbers in comparison to RCC4VHL. Thus, ROCK1 downregulation mimics the effect of Y-27632 treatment on viability of RCC4 cells, making it a likely target for Y-27632 causing synthetic lethality effect. Statistical analysis was performed using a paired t-test comparing numbers of colonies in each siROCK group to siControl. SEMs are shown. (b-c) The degree of each target knockdown by its specific siRNA (as indicated) was assessed by Western blot.