Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28774 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROCK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, HepG2, 293T, H1299, NIH-3T3, JC, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROCK2 Polyclonal Antibody (Product # PA5-28774). Sample (30 µg of whole cell lysate). Lane A: H1299. 5% SDS PAGE. ROCK2 Polyclonal Antibody (Product # PA5-28774) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROCK2 Polyclonal Antibody (Product # PA5-28774). Sample (30 µg of whole cell lysate). Lane A: H1299. 5% SDS PAGE. ROCK2 Polyclonal Antibody (Product # PA5-28774) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROCK2 Polyclonal Antibody (Product # PA5-28774). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. 5% SDS PAGE. ROCK2 Polyclonal Antibody (Product # PA5-28774) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROCK2 Polyclonal Antibody (Product # PA5-28774). Sample (50 µg of whole cell lysate). Lane A: Rat brain. 5% SDS PAGE. ROCK2 Polyclonal Antibody (Product # PA5-28774) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ROCK2 in methanol-fixed A431 cells using a ROCK2 polyclonal antibody (Product # PA5-28774) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human esophagous cancer, using ROCK2 (Product # PA5-28774) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.