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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09365PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09365PU-N, RRID:AB_2035925
- Product name
- anti ROCK2 pTyr256
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to residues surrounding Tyrosine 256 of Human ROCK-2
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-ROCK-2 pTyr256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution.
