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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA1-16946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is expected to react with rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of PHD2 in mouse kidney lysate using Product # PA1-16946. ECL exposure, 20 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHD2 in mouse kidney lysate. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16946). ECL exposure, 20 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PHD2 in mouse renal tubular epithelium. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16946) using a dilution of 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PHD2 in formalin-fixed paraffin-embedded mouse lung section. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16946) using a dilution of 1:200 followed by HRP conjugated anti-rabbit secondary antibody and DAB reagent. The antibody generated a specific staining in the cytoplasm and nuclei of alveolar as well as bronchiolar epithelial cells. Cytoplasmic staining was observed in almost all cells while the nuclear positivity was seen in a subset of cells only.
