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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-17050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 77 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHD-2/Egln1 in extracts SNB19 and Wek293 cells using PHD-2/Egln1 polyclonal antibody (Product # PA5-17050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PHD2 Polyclonal Antibody (Product # PA5-17050) and a 47-50kDa band corresponding to Egl nine homolog 1 was observed across all the cell lines and tissue models tested. Whole-cell extracts (30 µg lysate) of A549 (Lane 1), U-87 MG (Lane 2), U-2 OS (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), HeLa treated with cobalt chloride (200 micromolar for 48h) (Lane 6), Hep G2 (Lane 7), Mouse Heart (Lane 8), Mouse Brain (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, (1:4000 dilution)) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
