NB100-2219

antibody from Novus Biologicals

Targeting: EGLN1 C1orf12, HIFPH2, PHD2, SM-20, ZMYND6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2219

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2219, RRID:AB_2096717

Product name

Rabbit Polyclonal EGLN1/PHD2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span.

Laitakari A, Huttunen R, Kuvaja P, Hannuksela P, Szabo Z, Heikkilä M, Kerkelä R, Myllyharju J, Dimova EY, Serpi R, Koivunen P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):5590-5609

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Systemic silencing of PHD2 causes reversible immune regulatory dysfunction.

Yamamoto A, Hester J, Macklin PS, Kawai K, Uchiyama M, Biggs D, Bishop T, Bull K, Cheng X, Cawthorne E, Coleman ML, Crockford TL, Davies B, Dow LE, Goldin R, Kranc K, Kudo H, Lawson H, McAuliffe J, Milward K, Scudamore CL, Soilleux E, Issa F, Ratcliffe PJ, Pugh CW
The Journal of clinical investigation 2019 Jun 4;129(9):3640-3656

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Metallothionein 3 Controls the Phenotype and Metabolic Programming of Alternatively Activated Macrophages.

Chowdhury D, Alrefai H, Landero Figueroa JA, Candor K, Porollo A, Fecher R, Divanovic S, Deepe GS Jr, Subramanian Vignesh K
Cell reports 2019 Jun 25;27(13):3873-3886.e7

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Effects of empagliflozin on nondiabetic salt-sensitive hypertension in uninephrectomized rats.

Kim S, Jo CH, Kim GH
Hypertension research : official journal of the Japanese Society of Hypertension 2019 Dec;42(12):1905-1915

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Intermittent hypoxia induces beneficial cardiovascular remodeling in left ventricular function of type 1 diabetic rat.

Akat F, Fıçıcılar H, Durak A, Tuncay E, Dursun AD, Topal Çelikkan F, Sabuncuoğlu B, Turan B, Baştuğ M
Anatolian journal of cardiology 2018 Apr;19(4):259-266

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The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia.

Sim J, Cowburn AS, Palazon A, Madhu B, Tyrakis PA, Macías D, Bargiela DM, Pietsch S, Gralla M, Evans CE, Kittipassorn T, Chey YCJ, Branco CM, Rundqvist H, Peet DJ, Johnson RS
Cell metabolism 2018 Apr 3;27(4):898-913.e7

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Ablation of endothelial prolyl hydroxylase domain protein-2 promotes renal vascular remodelling and fibrosis in mice.

Wang S, Zeng H, Chen ST, Zhou L, Xie XJ, He X, Tao YK, Tuo QH, Deng C, Liao DF, Chen JX
Journal of cellular and molecular medicine 2017 Sep;21(9):1967-1978

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Chronic kidney disease leads to hypoxia inducible factor-1alpha to hypoxia inducible factor-2alpha switch in the gastrocnemius muscle.

Flisinski M, Wisniewska-Chudy E, Brymora A, Stefanska A, Strozecki P, Manitius J
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2017 Jun;68(3):419-425

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15-Deoxy-Δ^12,14-prostaglandin J₂ stabilizes hypoxia inducible factor-1α through induction of heme oxygenase-1 and direct modification ofprolyl-4-hydroxylase 2.

Choi JE, Kim JH, Song NY, Suh J, Kim DH, Kim SJ, Na HK, Nadas J, Dong Z, Cha YN, Surh YJ
Free radical research 2016 Oct;50(10):1140-1152

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HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury.

Karsikas S, Myllymäki M, Heikkilä M, Sormunen R, Kivirikko KI, Myllyharju J, Serpi R, Koivunen P
Journal of molecular medicine (Berlin, Germany) 2016 Mar;94(3):301-10

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LPS causes pericyte loss and microvascular dysfunction via disruption of Sirt3/angiopoietins/Tie-2 and HIF-2α/Notch3 pathways.

Zeng H, He X, Tuo QH, Liao DF, Zhang GQ, Chen JX
Scientific reports 2016 Feb 12;6:20931

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Prolyl hydroxylase domain-2 silencing induced by hydrodynamic limb vein injection enhances vascular regeneration in critical limb ischemia mice through activation of multiple genes.

Wu S, Zhang J, Huang C, Jia H, Wang Y, Xu Z, Yang L, Miyagishi M, Kasim V
Current gene therapy 2015;15(3):313-25

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Prolyl hydroxylase domain-2 silencing induced by hydrodynamic limb vein injection enhances vascular regeneration in critical limb ischemia mice through activation of multiple genes.

Wu S, Zhang J, Huang C, Jia H, Wang Y, Xu Z, Yang L, Miyagishi M, Kasim V
Current gene therapy 2015;15(3):313-25

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PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban.

Xie L, Pi X, Townley-Tilson WH, Li N, Wehrens XH, Entman ML, Taffet GE, Mishra A, Peng J, Schisler JC, Meissner G, Patterson C
The Journal of clinical investigation 2015 Jul 1;125(7):2759-71

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Epidermal or dermal specific knockout of PHD-2 enhances wound healing and minimizes ischemic injury.

Zimmermann AS, Morrison SD, Hu MS, Li S, Nauta A, Sorkin M, Meyer NP, Walmsley GG, Maan ZN, Chan DA, Gurtner GC, Giaccia AJ, Longaker MT
PloS one 2014;9(4):e93373

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Lights on for HIF-1α: genetically enhanced mouse cardiomyocytes for heart tissue imaging.

Hesse AR, Levent E, Zieseniss A, Tiburcy M, Zimmermann WH, Katschinski DM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2014;34(2):455-62

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Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.

Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski DM
Journal of leukocyte biology 2014 Sep;96(3):365-75

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Expression of hypoxia-inducible factor-1α, vascular endothelial growth factor and prolyl hydroxylase domain protein 2 in cutaneous squamous cell carcinoma and precursor lesions and their relationship with histological stages and clinical features.

An X, Xu G, Yang L, Wang Y, Li Y, McHepange UO, Shen G, Tu Y, Tao J
The Journal of dermatology 2014 Jan;41(1):76-83

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The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation.

Kim J, Kwak HJ, Cha JY, Jeong YS, Rhee SD, Cheon HG
The Journal of biological chemistry 2014 Jan 31;289(5):2755-64

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HIF-1α is upregulated in human mesenchymal stem cells.

Palomäki S, Pietilä M, Laitinen S, Pesälä J, Sormunen R, Lehenkari P, Koivunen P
Stem cells (Dayton, Ohio) 2013 Sep;31(9):1902-9

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Activation of hypoxia-inducible factor-1 in pulmonary arterial smooth muscle cells by endothelin-1.

Pisarcik S, Maylor J, Lu W, Yun X, Undem C, Sylvester JT, Semenza GL, Shimoda LA
American journal of physiology. Lung cellular and molecular physiology 2013 Apr 15;304(8):L549-61

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Overexpression of factor inhibiting HIF-1 enhances vessel maturation and tumor growth via platelet-derived growth factor-C.

Kuzmanov A, Wielockx B, Rezaei M, Kettelhake A, Breier G
International journal of cancer 2012 Sep 1;131(5):E603-13

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Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia.

Kunze R, Zhou W, Veltkamp R, Wielockx B, Breier G, Marti HH
Stroke 2012 Oct;43(10):2748-56

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Impaired hypoxic response in senescent mouse brain.

Rabie T, Kunze R, Marti HH
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2011 Oct;29(6):655-61

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Identification of human endogenous retrovirus-specific T cell responses in vertically HIV-1-infected subjects.

Tandon R, SenGupta D, Ndhlovu LC, Vieira RG, Jones RB, York VA, Vieira VA, Sharp ER, Wiznia AA, Ostrowski MA, Rosenberg MG, Nixon DF
Journal of virology 2011 Nov;85(21):11526-31

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Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury.

Hölscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, Wielockx B, Breier G, Katschinski DM, Zieseniss A
The Journal of biological chemistry 2011 Apr 1;286(13):11185-94

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Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injury.

Hyvärinen J, Hassinen IE, Sormunen R, Mäki JM, Kivirikko KI, Koivunen P, Myllyharju J
The Journal of biological chemistry 2010 Apr 30;285(18):13646-57

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HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury.

Schödel J, Klanke B, Weidemann A, Buchholz B, Bernhardt W, Bertog M, Amann K, Korbmacher C, Wiesener M, Warnecke C, Kurtz A, Eckardt KU, Willam C
The American journal of pathology 2009 May;174(5):1663-74

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Longitudinal and multimodal in vivo imaging of tumor hypoxia and its downstream molecular events.

Lehmann S, Stiehl DP, Honer M, Dominietto M, Keist R, Kotevic I, Wollenick K, Ametamey S, Wenger RH, Rudin M
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 18;106(33):14004-9

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Enhancement of Angiogenesis Through Stabilization of Hypoxia-inducible Factor-1 by Silencing Prolyl Hydroxylase Domain-2 Gene

Shourong Wu, Nobuhiro Nishiyama, Mitsunobu R Kano, Yasuyuki Morishita, Kohei Miyazono, Keiji Itaka, Ung-il Chung, Kazunori Kataoka
Molecular Therapy 2008 May;16(7):1227-1234

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Enhancement of angiogenesis through stabilization of hypoxia-inducible factor-1 by silencing prolyl hydroxylase domain-2 gene.

Wu S, Nishiyama N, Kano MR, Morishita Y, Miyazono K, Itaka K, Chung UI, Kataoka K
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Jul;16(7):1227-34

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The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress.

Mikhaylova O, Ignacak ML, Barankiewicz TJ, Harbaugh SV, Yi Y, Maxwell PH, Schneider M, Van Geyte K, Carmeliet P, Revelo MP, Wyder M, Greis KD, Meller J, Czyzyk-Krzeska MF
Molecular and cellular biology 2008 Apr;28(8):2701-17

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Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine.

Kariuki TM, Van Dam GJ, Deelder AM, Farah IO, Yole DS, Wilson RA, Coulson PS
Infection and immunity 2006 Jul;74(7):3979-86

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EGLN1/PHD2 Antibody [NB100-2219] - Detection of EGLN1/PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Staining of renal tubular epithelium in mouse using NB100-2219 at 2.5 ug/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of a FFPE mouse lung section using 1:200 dilution of EGLN1/PHD2 antibody. The staining was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The antibody generated a specific staining in the cytoplasm and nuclei of alveolar as well as bronchiolar epithelial cells. Cytoplasmic staining was observed in almost all cells while the nuclear positivity was seen in a subset of cells only.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of PHD-2 on healed wounded skin and adjacent normal unwounded skin of wild type, heterozygous K14-Cre/homozygous floxed PHD-2, and heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice at 400x magnification. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY licence.