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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005603-M09 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005603-M09, RRID:AB_1577767
- Product name
- MAPK13 monoclonal antibody (M09), clone 2B6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant MAPK13.
- Antigen sequence
NDKAAKSYIQSLPQTPRKDFTQLFPRASPQAADLL
EKMLELDVDRRLTAAQALTHPFFEPFRDPEEETEA
QQPFDDSLEHEKLTVDEWKQHIYKEIVNFSPIARK
DSRRRSGMKL- Isotype
- IgG
- Antibody clone number
- 2B6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MAPK13 expression in transfected 293T cell line by MAPK13 monoclonal antibody (M09), clone 2B6.Lane 1: MAPK13 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MAPK13 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of MAPK13 transfected lysate using anti-MAPK13 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAPK13 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
