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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ABIN359420 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase 13 (MAPK13) (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MAPK13.
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- Middle Region
- Vial size
- 0.1 mg
- Storage
- Store the antibody at 2 - 8°C up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Evidence for a role of p38 kinase in hypoxia-inducible factor 1-independent induction of vascular endothelial growth factor expression by sodium arsenite.
Novel protein kinase C isoforms regulate human keratinocyte differentiation by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha.
A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta.
Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokines.
Identification of stathmin as a novel substrate for p38 delta.
Duyndam MC, Hulscher ST, van der Wall E, Pinedo HM, Boven E
The Journal of biological chemistry 2003 Feb 28;278(9):6885-95
The Journal of biological chemistry 2003 Feb 28;278(9):6885-95
Novel protein kinase C isoforms regulate human keratinocyte differentiation by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha.
Efimova T, Deucher A, Kuroki T, Ohba M, Eckert RL
The Journal of biological chemistry 2002 Aug 30;277(35):31753-60
The Journal of biological chemistry 2002 Aug 30;277(35):31753-60
A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta.
Knebel A, Morrice N, Cohen P
The EMBO journal 2001 Aug 15;20(16):4360-9
The EMBO journal 2001 Aug 15;20(16):4360-9
Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokines.
Hu MC, Wang YP, Mikhail A, Qiu WR, Tan TH
The Journal of biological chemistry 1999 Mar 12;274(11):7095-102
The Journal of biological chemistry 1999 Mar 12;274(11):7095-102
Identification of stathmin as a novel substrate for p38 delta.
Parker CG, Hunt J, Diener K, McGinley M, Soriano B, Keesler GA, Bray J, Yao Z, Wang XS, Kohno T, Lichenstein HS
Biochemical and biophysical research communications 1998 Aug 28;249(3):791-6
Biochemical and biophysical research communications 1998 Aug 28;249(3):791-6
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-MAPK13 Antibody (Center) (AP13904PU-N) in A2058 cell line lysates (35 μg/lane). MAPK13 (arrow) was detected using the purified Pab.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- The anti-MAPK13 Pab (AP13904PU-N) is used in Western blot to detect MAPK13 in mouse spleen tissue lysate.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
