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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- AF1947 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MSPR/Ron Antibody
- Antibody type
- Polyclonal
- Description
- Antigen and protein A Affinity-purified. The antibody detects endogenous human MSP R/Ron phosphorylated at Y1238/Y1239 using Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer.
Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-β in gastric tissue.
The Ron receptor tyrosine kinase positively regulates angiogenic chemokine production in prostate cancer cells.
Graves-Deal R, Bogatcheva G, Rehman S, Lu Y, Higginbotham JN, Singh B
Oncotarget 2019 Feb 12;10(13):1320-1333
Oncotarget 2019 Feb 12;10(13):1320-1333
Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-β in gastric tissue.
Ota M, Horiguchi M, Fang V, Shibahara K, Kadota K, Loomis C, Cammer M, Rifkin DB
Cancer research 2014 May 1;74(9):2642-51
Cancer research 2014 May 1;74(9):2642-51
The Ron receptor tyrosine kinase positively regulates angiogenic chemokine production in prostate cancer cells.
Thobe MN, Gurusamy D, Pathrose P, Waltz SE
Oncogene 2010 Jan 14;29(2):214-26
Oncogene 2010 Jan 14;29(2):214-26
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot. Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947).
