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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [2]
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- Product number
- MA5-11460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALK Monoclonal Antibody (5A4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-11460 targets ALK (Anaplastic Lymphoma Kinase) / p80 in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- 5A4
- Concentration
- Conc. Not Determined
Submitted references Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma.
Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.
The EML4-ALK transcript but not the fusion protein can be expressed in reactive and neoplastic lymphoid tissues.
Mutation-independent anaplastic lymphoma kinase overexpression in poor prognosis neuroblastoma patients.
Chen Q, Gu Y, Liu B
International journal of clinical and experimental pathology 2015;8(1):1007-12
International journal of clinical and experimental pathology 2015;8(1):1007-12
Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.
Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher RF, Cabassa P, Facchetti F
International journal of surgical pathology 2014 Dec;22(8):712-21
International journal of surgical pathology 2014 Dec;22(8):712-21
The EML4-ALK transcript but not the fusion protein can be expressed in reactive and neoplastic lymphoid tissues.
Sozzi G, Martelli MP, Conte D, Modena P, Pettirossi V, Pileri SA, Falini B
Haematologica 2009 Sep;94(9):1307-11
Haematologica 2009 Sep;94(9):1307-11
Mutation-independent anaplastic lymphoma kinase overexpression in poor prognosis neuroblastoma patients.
Passoni L, Longo L, Collini P, Coluccia AM, Bozzi F, Podda M, Gregorio A, Gambini C, Garaventa A, Pistoia V, Del Grosso F, Tonini GP, Cheng M, Gambacorti-Passerini C, Anichini A, Fossati-Bellani F, Di Nicola M, Luksch R
Cancer research 2009 Sep 15;69(18):7338-46
Cancer research 2009 Sep 15;69(18):7338-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK antibody using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALK/CD246 showing staining in the cytoplasm and membrane of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a ALK/CD246 Mouse Monoclonal Antibody (Product # MA5-11460) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
