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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-16320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALK Monoclonal Antibody (SP8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is anaplastic lymphoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP8
- Vial size
- 500 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references High anaplastic lymphoma kinase immunohistochemical staining in neuroblastoma and ganglioneuroblastoma is an independent predictor of poor outcome.
Duijkers FAM, Gaal J, Meijerink JPP, Admiraal P, Pieters R, de Krijger RR, van Noesel MM
The American journal of pathology 2012 Mar;180(3):1223-1231
The American journal of pathology 2012 Mar;180(3):1223-1231
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of ALK/p80 using anti-ALK/p80 Monoclonal Antibody (Product # MA5-16320) in Anaplastic Lymphoma. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
