Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-37630 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-LIMK2 (Thr505) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency.
Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment.
Neganova I, Cotts L, Banks P, Gassner K, Shukurov A, Armstrong L, Ladds G, Lako M
Stem cells (Dayton, Ohio) 2019 Mar;37(3):318-331
Stem cells (Dayton, Ohio) 2019 Mar;37(3):318-331
Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment.
Wong WY, Pier M, Limesand KH
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Oct 1;315(4):R656-R667
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Oct 1;315(4):R656-R667
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Hela cells, untreated or treated with PMA, using LIMK2 (pThr505) polyclonal antibody (Product # PA5-37630).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-LIMK2 (Thr505) in methanol-fixed Hela cells, using Phospho-LIMK2 (Thr505) Polyclonal Antibody (Product # PA5-37630).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-LIMK2 (Thr505) in paraffin-embedded Human breast carcinoma tissue using Phospho-LIMK2 (Thr505) Polyclonal Antibody (Product # PA5-37630) (left) or the same antibody preincubated with blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7. Effect of Rho-associated coiled-coil containing kinase (ROCK)-inhibitor Y-27632 on E-cadherin/beta-catenin association after radiation treatment. Female FVB mice (4-6 wk old) were dissected and cultured as primary cell cultures. On day 0 , the primary cells were irradiated with 5 Gy and cell lysates were collected on days 1 , 2 , and 3 after radiation treatment. Experimental timeline ( A ). beta-catenin was immunoprecipitated from cell culture lysates and the level of E-cadherin bound to beta-catenin was determined by coimmunoprecipitation after radiation treatment ( B ). Cell cultures were either untreated (UT), untreated plus ROCK-inhibitor Y-27632 on day 2 (UT + Y-27632), irradiated (IR), or irradiated plus ROCK inhibitor Y-27632 on day 2 (IR + Y-27632; C ). Cell lysates were collected on day 3 after radiation treatment for all conditions. Phosphorylated Lim domain kinase (pLIMK) was immunoblotted in UT, UT + Y-27632, IR, and IR + Y-27632 condition ( C ). Immunoblots were reprobed for total levels of LIMK2 as a loading control. beta-catenin was immunoprecipitated from cell lysates and the level of E-cadherin bound to beta-catenin was determined by immunoblot ( D ). Quantification by densitometry of D , normalized to UT. Results are presented from three independent primary cell preparations ( E ); error bars denote means +- SE. Significant difference ( P < 0.05) was determined by ANOVA followed by a post hoc Tukey's multiple comparison test